Researchers at Tokyo University have published new data on Asian research
2009 JUN 25 - (VerticalNews.com) -- "TLC profiles of aminolipids extracted from phytopathogenic and non-pathogenic bacteria with chloroform-methanol-0.3%NaCl (2:1:0.2, v/v/v) or 2-propanol are useful for discrimination of bacteria. For many bacteria, each TLC profile is genus or species specific and highly reproducible," investigators in Atsugi, Japan report.
"For most gram-negative bacteria, the uppermost spot (Up) appeared at ca. R-f 0.7 on the chromatograms developed with chloroform-methanol-0.2% CaCl2 center dot 2H(2)O (55:35:8, v/v/v). This spot was absent on the chromatograms of grain-positive bacteria, Clavibacter michiganeasis. The profiles of Agrobacterium spp. and Rhizobium spp. were different from other gram-negative bacteria with the uppermost spots at ca. R-l 0.75. For the case of Agrobacterium spp., the chromatograms of the strains belonging to the same biovar were identical. Distinct differences were found among the profiles of Agrobacterium, biovar 1, A. biovar 2, A. biovar 3 and A. rubi. The profiles of Rhizobium. spp., except for R. tropici, and their relatives such as Bradyrhizobium, Mesorhizobium and Sinorhizobium spp. were quite simple and different from those of Agrobacterium spp. For the case of Burkholderia, species, except. for B. andropogonis, three spots (designated as S1, S2, S3) appeared under the uppermost spot (Up) and their profiles were species specific for several species such as B. plantarii and B. caryophylli. On the chromatogram of R. andropogonis, the S1 spot, (non-phosphorous) was absent and the S3 spot was faint. The profiles of 96 Ralstonia solanacearum. strains from various Sources were identical. For the case of Erwinia carotovora an intensive benchmark spot, appeared at. R, 0,64 but this spot was absent on the chromatograms of pathovars of E. chrysavthemi and F. herbirola. Clear diversity in profiles was observed between Xanthomonas campestris and X. oryzae. The profile of pathovars of Pseudomonas syringae were identical and simple," wrote N. Matsuyama and colleagues, Tokyo University.
The researchers concluded: "Substitution of chloroform solvent, systems with less hazardous organic solvents,vas tested. 2-propanol for the lipid extraction and 1-butanol-acetic acid-water (3:1:1 and 5:3:1, v/v/v) for the developing solvents were usable, though development with the butanol systems was highly time-consuming."
Matsuyama and colleagues published their study in the Journal of the Faculty of Agriculture Kyushu University (Presumptive Differentiation of Phytopathogenic and Non-pathogenic Bacteria by Improved Rapid-Extraction TLC Method. Journal of the Faculty of Agriculture Kyushu University, 2009;54(1):1-11).
For additional information, contact N. Matsuyama, Tokyo University Agriculture, Faculty Agriculture, Atsugi, Kanagawa 2430034, Japan.
The publisher of the Journal of the Faculty of Agriculture Kyushu University can be contacted at: Kyushu University, Faculty Agricultural Publications, 6-10-1 Hakozaki, Higashi-Ku, Fukuoka-Shi, 812-0053, Japan.
Keywords: Agriculture, Tokyo University.
This article was prepared by VerticalNews Agriculture editors from staff and other reports. Copyright 2009, VerticalNews Agriculture via VerticalNews.com.