New Agribusiness Research Reported from Banaras Hindu University
2012 MAY 10 - (VerticalNews.com) -- "An improved and efficient in vitro regeneration system has been developed for Eclipta alba, a medicinally important plant, through transverse thin cell layer culture (tTCL). The transverse section of the nodal segment of field grown plants was used as tTCL explants for plant regeneration," scientists in Uttar Pradesh, India report.
"Shoot multiplication from tTCL nodal explants was influenced by BAP and their interaction with Kin or NAA. MS medium containing 13.2 mu M BAP and 4.6 mu M Kin was most effective for shoot multiplication from tTCL nodal explants. Upon this medium, percent response for shoot proliferation was 100% with an average of 32.6 shoot buds per tTCL nodal explant. Regenerated shoots from tTCL nodal explants were rooted on the growth regulator free MS medium. The rooted plantlets were successfully acclimatized and established in soil with a survival frequency of 90-100%. Random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic fidelity of the micropropagated plants," wrote S.K. Singh and colleagues, Banaras Hindu University.
The researchers concluded: "RAPD profile analysis indicated that micropropagated plants were genetically similar to mother plant."
Singh and colleagues published their study in Industrial Crops and Products (An improved and efficient micropropagation of Eclipta alba through transverse thin cell layer culture and assessment of clonal fidelity using RAPD analysis. Industrial Crops and Products, 2012;37(1):328-333).
For more information, contact S.K. Singh, Banaras Hindu Univ, Dept. of Bot, Lab Morphogenesis, Varanasi 221005, Uttar Pradesh, India.
Publisher contact information for the journal Industrial Crops and Products is: Elsevier Science Bv, PO Box 211, 1000 Ae Amsterdam, Netherlands.
Keywords: City:Uttar Pradesh, Country:India, Region:Asia, Agribusiness
This article was prepared by VerticalNews Agriculture editors from staff and other reports. Copyright 2012, VerticalNews Agriculture via VerticalNews.com.